Considering nutritional benefits of cucumbers in human diet, the crop is protected against biotic stress. Based on the 16Sr RNA gene analysis, 16 different ribosomal groups and subgroups phytoplasmas infecting different vegetable crops have been well documented across the world ( Kumari et al. 2021).The phytoplasma is one of the emerging pathogens in many vegetable crops and causing huge loss to various crops. 2007), Trichosanthes cucumerina ( Weng et al. 2017), Sechium edule (Jacquin) Swartz ( Villalobos et al. 2015) and cucumber yellows (Valiunas et al. 2017), cucumber phyllody in Iran (Hosseini et al. Many cucurbits belongs to the Cucurbitaceous family have been infected with different phytoplasma has been well documented in different parts of the world that includes witches’ broom of Cucurbita pepo in India ( Rao et al. All over the world, phytoplasmas cause a multitude of diseases in several hundred crop species including fruits, vegetables, cereals and trees ( McCoy et al. 2019).Ĭucumber is reported to be attacked by plethora of viruses, bacteria and fungi in India, but not yet by phytoplasmas. The cultivation of cucumber is threaten by number insects and diseases from germination till harvest and causes heavy economic losses to the growers ( Venkataravanappa et al. The cucumber is mainly gown during spring-summer and rainy season in commercial scale as well as kitchen gardens. In India cucumber is cultivated with in an area of 78,000 ha with the production of 1,142,000 MT (Anonymous, 2018 NHM data base). The crop is extensively cultivated for its edible tender fruit, preferred as salad ingredient, pickles and also as cooked vegetable ( Navitha et al. It is the world’s ancient vegetable crop originated in India ( Yamaguchi 1983) and popularly known as ‘khira’ and gherkins. This is the first report on phytoplasma associated with phyllody disease of cucumber in India.Ĭucumber ( Cucumis sativus L.) is an important vegetable crop belongs to family Cucurbitaceae grown widely in warm regions of the world. Further on the basis of computer-simulated RFLP (= in silico RFLP) analysis of amplified F2n/R2 region of 16S rRNA gene indicates that, the detected phytoplasma was belongs to the subgroup X (16SrII-X). Sequence and phylogenetic analyses of 16S rRNA, secY and rp genes revealed that the detected phytoplasma is a member of the 16SrI group ( Candidatus Phytoplasma asteris). The PCR amplified products 16SrRNA (1.2 kb), SecY (1.6 kb) rp gene (1.2 kb) was cloned and sequenced. After general detection the non-ribosomal SecY and rp (ribosomal protein) genes was amplified using specific primers. The etiology of the cucumber phyllody phytoplasma (CuPP) was confirmed by amplifying 16S rRNA gene from symptomatic plants using PCR followed by nested PCR using universal primers pairs P1/P7 and R16F2n/R16R2. Cucumber ( Cucumis sativus) plants exhibiting typical phyllody symptoms were collected from farmers field of Chintamani, Chickballapur districts of Karnataka (India).
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